BPS2025 Full Program

TMEM175, TPC2 and TRPML1. Measurements of K + and H + permeability in TMEM175, revealed two K+ permeability coefficients and a PH/PK ratio consistent with literature. For TPC2, saturable Na+ conductance with an EC50 of ~40 mM was observed, enhanced by the potentiator TPC2-A1P. SSME offers valuable insights into lysosomal channel func tion, complementing traditional patch-clamp techniques. Integrated Optical and Electrical Techniques for Investigating Unsupported Lipid Bilayer Dynamics at the Single-Molecule Level Speaker: Dan Burden, Professor, Analytical Chemistry, Wheaton College Chemistry Department This presentation examines the microelectrode cavity array (MECA) plat form for studying biomolecule dynamics in lipid bilayers, emphasizing simultaneous optical and electrical measurements. The platform detects single fluorescent molecules translocating across lipid bilayers while monitoring electrical currents typical of ion-channel experiments. This dual-modal approach provides insights into membrane processes at the single-molecule level, enabling the study of electrically silent transloca tion events often missed with conventional methods. Dr. Burden will showcase the method’s utility for analyzing lipid and protein diffusion, ion channel insertion, gating, and the influence of biomimetic structures on membrane properties. Key techniques include fluorescence correla tion spectroscopy (FCS) and single-particle tracking (SPT). Additionally, challenges with single-molecule fluorescence, such as optical surface irregularities, will be addressed, along with strategies for minimizing background fluorescence and improving signal-to-noise ratios to maxi mize research potential. Biophysical Journal Associate Editor Meeting 11:30 am - 1:30 pm, Room 405 Career Development Center Workshop Marketing Your Value: Crafting your Elevator Pitch/30 Second Value Statement/Brand Statement 12:00 pm - 1:00 pm, Room 409B How to effectively identify and communicate your value to decision makers, including during networking, conference participation, and the job search process. We will delve into crafting the elevator pitch and key and clever strategies for amplifying your reputation. Exhibitor Presentation Leica Microsystems 12:30 pm - 2:00 pm, Room 406AB Advances in Label-Free Chemical Imaging with Stimulated Raman Scat tering Microscopy The application space of stimulated Raman scattering (SRS) microscopy in life sciences research continues to expand rapidly. Ongoing advances in laser technology are resulting in dramatic increases in acquisition speeds, particularly for multi-color or hyperspectral datasets, facilitating the generation of statistically relevant label-free image datasets. Here, we present the STELLARIS CRS – Leica’s turnkey Coherent Raman Scattering system. It offers the two popular CRS modalities – Stimulated Raman Scattering (SRS) and Coherent Anti-Stokes Raman Scattering (CARS) – and allows for the simultaneous acquisition of two-photon fluorescence and second-harmonic generation signals. Importantly, the seamless integration of CRS with the STELLARIS visible confocal fluores cence microscopy platform, as well as Fluorescence Lifetime Imaging, results in a multi-modal optical discovery instrument capable of captur ing a unique combination of biochemical, biophysical and molecular contrasts.

We will present a range of new applications of SRS microscopy that capitalize on these advances. We highlight new capacities in label-free, multi-modal metabolic profiling at the single-cell level, as well as high speed, multi-color, label-free chemical imaging of dynamic biological processes. Our results emphasize the growing ease-of-use of SRS mi croscopy, and its capacity to contribute to an even deeper understand ing of cell and tissue biology, serving as a powerful new tool for a wide range of research in the life sciences. Speakers Haridas Pudavar, Product Performance Manager, Leica Microscopy Inc Volker Schweikhard, Application Scientist, Advanced Microscopy, Leica Microscopy Inc Artificial Intelligence Policy in Biomedical Research 1:00 pm - 2:30 pm, Room 408A The Advanced Research Projects Agency for Health (ARPA-H) will be entering its second full year of operation. Join the Public Affairs Commit tee (PAC) as we learn more about the fledgling high-risk, high-reward re search agency, how the structure will continue to form, exploring what opportunities exist and how New policies and regulations on artificial intelligence (AI) are emerging at a rapid pace in Washington, DC. Join a panel hosted by the Public Affairs Committee as we explore the impacts of White House Executive Orders on AI and pending legislative efforts impacting AI use in scientific research. Moderators Ana Nicoleta Bondar, University of Bucharest, Romania Speakers Kavita M Berger, Board on Life Sciences National Academies of Science, USA Stephanie Batalis, Center for Security and Emerging Technology (CSET), USA Grant Rotskoff, Stanford University, USA Career Development Center Workshop Looking Beyond Academia: Identifying Your Career Options using MyIDP, LinkedIn and More 1:15 pm - 2:15 pm, Room 409B Not sure where your professional future lies or how to approach the process in an organized and strategic manner? This presentation pro vides a framework and resources for moving forward with confidence towards the next step in your professional future. In addition, it will provide specific examples of how to build out your knowledge of a new potential career field and forge valuable connections that can facilitate a successful transition. Biophysics 101: Wearable Technology 1:30 pm - 3:00 pm, Room 502A Biophysics 101 is an opportunity to learn about hot topics in Biophysics from experts in the field! This year, two expert speakers will introduce the field of wearable technology along with computational imaging and sensing, including how biophysics contributes to the development of these systems and their diverse applications for human health. Moderators Jefferson Knight, University of Colorado - Denver, USA Andrea Ghisleni, IFOM - the FIRC Institute of Molecular Oncology, Italy Elizabeth Schneider, University of Tennessee, USA Speakers Aydogan Ozcan, University of California, Los Angeles, USA Muyang Lin, University of California, San Diego, USA

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