Biophysical Society 62nd Annual Meeting | Program Guide

588-Pos Board B358 THE RYR2 R420Q MUTATION TRIGGERS CATECHOLAMINERGIC POLYMOR- PHIC VENTRICULAR TACHYCARDIA IN MOUSE CARDIOMYOCYTES VIA SR CALCIUM LOADING. Miguel Fernandez-Tenorio , Radoslav Janicek, Riccardo Rizzetto, Alexandra Zahradnikova Jr., Yue Yi Wang, Jean-Pierre Benitah, Esther Zorio, Ana M. Gomez, Ernst Niggli 589-Pos Board B359 DUAL ABLATION OF THE RYR2-S2808 AND RYR2-S2814 SITES INCREASES PROPENSITY FOR PRO-ARRHYTHMIC SPONTANEOUS CALCIUM RELEAS- ES. Duilio Michele Potenza , Radoslav Janiček, Miguel Fernandez-Tenorio, Hector H. Valdivia, Ernst Niggli 590-Pos Board B360 SEARCH FOR TYPE 2 RYANODINE RECEPTOR INHIBITOR BY MONITORING ENDOPLASMIC RETICULUM CA 2+ . Mai Tamura , Nagomi Kurebayashi, Ta- kashi Murayama, Shuichi Mori, Mari Ishigami-Yuasa, Hiroyuki Kagechika, Junji Suzuki, Kazunori Kanemaru, Masamitsu Iino, Takashi Sakurai 591-Pos Board B361 REGULATION OF STORE CA 2+ LEVEL IN HEK293 CELLS EXPRESSING TYPE 2 RYANODINE RECEPTOR (RYR2) WITH CPVT MUTATIONS.  Nagomi Kurebayashi , Takashi Murayama, Ryosaku Ohta, Fumiyoshi Yamashita, Junji Suzuki, Kazunori Kanemaru, Masamitsu Iino, Takashi Sakurai 592-Pos Board B362 THE MECHANISM OF FLECAINIDE ACTION IN CPVT INVOLVES A DIRECT EFFECT ON RYR2. Dmytro O. Kryshtal , Daniel J. Blackwell, Nieves Gomez- Hurtado, Suzanne M. Batiste, Jeffrey N. Johnston, Björn C. Knollmann 593-Pos Board B363 NOVEL INHIBITORS OF LEAKY SKELETAL RYANODINE RECEPTOR CALCIUM CHANNELS DISCOVERED VIA FRET-BASED HIGH-THROUGHPUT SCREEN- ING. Claire E. Haskin , Robyn T. Rebbeck, Gregory D. Gillispie, David D. Thomas, Razvan L. Cornea 594-Pos Board B364 A FUNCTIONAL SIGNIFICANCE OF amINO-TERMINAL CYSTEINES OF RYR2. Roman Nikolaienko , Elisa Bovo, Daniel Kahn, Aleksey Zima 595-Pos Board B365 HIGH-THROUGHPUT SCREENING YIELDS ALLOSTERIC INHIBITORS OF LEAKY RYRS FOR THERAPEUTIC APPLICATIONS. Robyn T. Rebbeck, Xiaoq- iong Dong, Kenneth S. Ginsburg, Daniel P. Singh, Gregory D. Gillispie, Da- vid D. Thomas, Bradley S. Launikonis, Donald M. Bers, Razvan L. Cornea 596-Pos Board B366 CRISPR/CAS9 CREATED CPVT1 ASSOCIATED RYR2 MUTATIONS RELIABLY REPRODUCES THE CALCIUM SIGNALING ABERRANCY IN HUMAN IPSC- CMS. Xiaohua Zhang , Hua Wei, Naohiro Yamaguchi, Martin Morad 597-Pos Board B367 MOLECULAR SCALE VISUALISATION OF VARIABLE CLUSTERING PROPER- TIES OF THE CARDIAC RYANODINE RECEPTOR. ID Jayasinghe, AH Clowsley, R Lin, T Lutz, Carl Harrison, EM Green, D Baddeley, L Di Michele, C Soeller 598-Pos Board B368 MOLECULES TO MEMBRANES: THE CALCIUM RELEASE UNIT.  Sophia P. Hirakis , Thomas M. Bartol, Terrence J. Sejnowski, Rommie E. amaro 599-Pos Board B369 USING STATISTICAL MECHANICS TO UNDERSTAND CALCIUM SPARKS. Anna V. Maltsev , Michael D. Stern, Victor A. Maltsev 600-Pos Board B370 A NOVEL COMPUTATIONAL MODEL OF THE RABBIT ATRIAL MYOCYTE OF- FERS INSIGHT INTO CALCIUMWAVE PROPAGATION FAILURE.  Marcia R. Vagos , Jordi Heijman, Hermenegild Arevalo, Mary M. Maleckar, Bernardo Lino de Oliveira, Ulrich Schotten, Joakim Sundnes

601-Pos Board B371 THE MODELING OF CALCIUM DYNAMICS WITHIN THE DYADIC SPACE US- ING RANDOMWALKS.  Jessica Au , Zana Coulibaly, Leighton Chen, Daisuke Sato 602-Pos Board B372 MOLECULAR CONTACTS BETWEEN MCU AND ITS REGULATORY MACHIN- ERY. Charles Phillips 603-Pos Board B373 THE FUNCTION OF MICU2 IN MITOCHONDRIAL CALCIUM UNIPORT.  Kai-Ting Huang , Melaine Paillard, Peter Varnai, Gyorgy Hajnoczky 604-Pos Board B374 MICU2 RESTRICTS SPATIAL CROSSTALK BETWEEN INSP 3 R AND MCU CHANNELS BY REGULATING THRESHOLD AND GAIN OF MICU1-MEDIATED INHIBITION AND ACTIVATION OF MCU. Riley Payne , Henry Hoff, Anne Roskowski, J. Kevin Foskett Voltage-gated K Channels and Mechanisms of Voltage Sensing and Gating I (Boards B375–B397) 605-Pos Board B375 INVESTIGATING CYCLIC DINUCLEOTIDE BINDING TO HCN CHANNELS BY SURFACE PLASMON RESONANCE AND ISOTHERMAL CALORIMETRY. Se- bastien Hayoz, Purushottam B. Tiwari, Grzegorz Piszczek, Aykut Üren, Tinatin I. Brelidze 606-Pos Board B376 EXAMINING DRUG BINDING IN HCN CHANNELS.  Jeremie Tanguay , Naz- zareno D’avanzo 607-Pos Board B377 MOLECULAR INTERACTIONS THAT CONTRIBUTE TO THE REGULATION OF HCN CHANNELS BY KCNE2. Yoann Lussier , Karen Callahan, Rikard Blunck, Nazzareno D’Avanzo 608-Pos Board B378 TOWARDS REVEALING A COOPERATIVE MECHANISM OF CAMP BINDING TO HCN2 CYCLIC NUCLEOTIDE BINDING DOMAINS AT THE SINGLE-MOL- ECULE LEVEL. David S. White , Marcel P. Goldschen-Ohm, Ruohan Zhang, Vadim A. Klenchin, Randall H. Goldsmith, Baron Chanda 609-Pos Board B379 ROLE OF INTERACTIONS BETWEEN TRANSMEMBRANE AND C-TERMINAL REGIONS IN VOLTAGE-DEPENDENT ACTIVATION OF HCN4 CHAN- NELS. Dana A. Page , Kaylee E. Magee, Jessica Li, Edgar C. Young 610-Pos Board B380 ETHANOL INCREASES NEURONAL FIRING BY REGULATING PI(4,5)P 2 SENSI- TIVITY OF M-TYPE K + CHANNELS. Kwon-Woo Kim , Byung-Chang Suh 611-Pos Board B381 MOLECULAR BASIS OF VOLTAGE ACTIVATION OF AN EPILEPSY-CAUSING MUTATION IN THE S4 OF KCNQ3 CHANNEL. Rene Barro-Soria 612-Pos Board B382 USE- AND STATE-DEPENDENT BINDING OF KCNQ CHANNEL OPEN- ERS. Caroline K. Wang , Harley T. Kurata, Alice W. Wang 613-Pos Board B383 EFFECT OF CAMP WHEN VARYING THE NUMBER OF KCNE1 SUBUNITS IN THE IKS COMPLEX. Emely Thompson , Jodene Eldstrom, Maartje Westhoff, Donald McAfee, David Fedida 614-Pos Board B384 MOLECULAR MECHANISM OF POLYUNSATURATED FATTY ACID ANA- LOGUES AS KV7.1-CHANNEL MODULATORS. Sara I. Liin , Rosamary Ramentol, Rene Barro-Soria, H. Peter Larsson

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