Biophysical Society 62nd Annual Meeting | Program Guide

981-Plat 8:30 am STRUCTURAL ANALYSIS OF A HUMAN MITOTIC KINESIN AND ITS POTEN- TIAL BINDING SITE FOR A SMALL MOLECULE INHIBITOR.  Hee-Won Park, Zhujun Ma, Haizhong Zhu, Shimin Jiang, Robert C. Robinson, Sharyn A. Endow 982-Plat 8:45 am CPOW Travel Awardee NATIVE KINESIN-1 DOES NOT PREFERENTIALLY BIND TO GTP-RICH MICRO- TUBULES IN VITRO.  Qiaochu Li, Stephen J. King, Jing Xu 983-Plat 9:00 am DIFFERENTIAL PHOSPHORYLATION IN THE MOTOR DOMAIN OF MITOTIC KINESIN-5 CIN8 REGULATES ITS FUNCTIONS IN VIVO .  Nurit Siegler , Alina Goldstein, Ofer Shapira, Darya Goldman, Ervin Valk, Mardo Kõivomägi, Mart Loog, Larisa Gheber 984-Plat 9:15 am KINESIN-8 DEPOLYMERIZES MICROTUBULES WITH A FORCE-DEPENDENT MECHANISM.  Anita Jannasch , Michael Bugiel, Erik Schäffer 985-Plat 9:30 am HOOK1 INDUCES SUPERPROCESSIVE MOTILITY OF DYNEIN AND IS REQUIRED FOR TRAFFICKING OF SIGNALING ENDOSOMES IN NEURONS.  Mara A. Olenick , Erika L.F. Holzbaur 986-Plat 9:45 am MICROTUBULE ASSOCIATED PROTEINS AND BUNDLING REGULATE KINESIN AND DYNEIN PROCESSIVITY AND FORCE GENERATION TO DIRECT INTRACELLULAR TRAFFICKING.  Abdullah R. Chaudhary, Linda Balabanian, Florian Berger, Christopher L. Berger, Adam G. Hendricks 987-Plat 10:00 am THE INTERPLAY OF DIFFUSION, MOTOR-DRIVEN WALKS, AND TETHERING IN INTRACELLULAR TRANSPORT.  Saurabh Mogre , Elena Koslover Exhibitor Presentation TA Instruments – Waters LLC 8:30 am–10:00 am, Exhibit Hall, Room 6 Characterizing Biopharmaceuticals for Stability and Affinity We will be discussing native and multi-parameter approaches to testing biopharmaceuticals. Isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) are powerful tools for in-depth characteriza- tion of molecular binding events and structural stability of biopharma- ceuticals. DSC and ITC generate comprehensive thermodynamic profiles for protein domain structures and the energetics of inter- and intra- molecular binding events. In addition to these stability and affinity assays, we have a new technique for determination of longer-term stability. Using an isothermal calorimeter, we can quantify shelf-life stability while simultaneously determining the percent aggregated material. This test is typically completed in a few days and has been shown to agree with longer-term SEC data. Speakers Colette Quinn, Microcalorimetry Product Manager, TA Instruments – Waters LLC Malin Suurkuusk, Isothermal Calorimetry Product Manager, TA Instruments – Waters LLC CPOW Committee Meeting 8:30 am–10:30 am, South, Level Three, Room 306

972-Plat 8:15 am NANOPORE TWEEZERS REVEAL DETAILED RNA POLYMERASE DYNAM- ICS AT A SEQUENCE-SPECIFIC PAUSE ELEMENT.  Ian C. Nova , Abhishek Mazumder, Jonathan M. Craig, Andrew H. Laszlo, Ian M. Derrington, Matthew T. Noakes, Henry Brinkerhoff, Jasmine Bowman, Jonathan W. Mount, Benjamin I. Tickman, Richard H. Ebright, Jens H. Gundlach 973-Plat 8:30 am SLIDING OF LAC REPRESSOR ALONG DNA IS SEQUENCE-DEPENDENT AND ALLOSTERICALLY REGULATED.  Alessia Tempestini, Carina Monico, Lucia Gardini, Francesco Vanzi, Francesco S. Pavone, Marco Capitanio 974-Plat 8:45 am SINGLE-MOLECULE CHARACTERIZATION OF P53 ON DNA USING DNA ARRAY “DNA GARDEN”.  Kiyoto Kamagata 975-Plat 9:00 am STRUCTURAL CHARACTERIZATION OF THE HIV-1 REVERSE TRANSCRIP- TASE INITIATION COMPLEX.  Kevin Larsen , Yamuna Mathiharan, Kalli Kappel, Aaron Coey, Dong-Hua Chen, Lauren Madigan, Georgios Skiniotis, Joseph Puglisi, Elisabetta Viani Puglisi 976-Plat 9:15 am CRISPR CAS9 MEDIATED DNA UNWINDING DETECTED USING SITE-DI- RECTED SPIN LABELING.  Narin S. Tangprasertchai, Rosa Di Felice, Xiaojun Zhang, Ian M. Slaymaker, Carolina Vazquez Reyes, Wei Jiang, Remo Rohs, Peter Qin 977-Plat 9:30 am ENHANCED PROOFREADING GOVERNS CRISPR-CAS9 TARGETING AC- CURACY.  Janice S. Chen, Yavuz S. Dagdas , Benjamin P. Kleinstiver, Moira M. Welch, Alexander A. Sousa, Lucas B. Harrington, Samuel H. Sternberg, Keith J. Joung, Ahmet Yildiz, Jennifer A. Doudna 978-Plat 9:45 am INVESTIGATING AND MODELLING THE TARGET RECOGNITION DYNAMICS OF THE CRISPR-CAS SURVEILLANCE COMPLEX CASCADE.  Marius Rutkauskas , Tomas Sinkūnas, Inga Songailiene, Virginijus Siksnys, Ralf Seidel 979-Plat 10:00 am DIRECT OBSERVATION OF TYPE IA TOPOISOMERASE GATE OPENING.  Maria Mills , Yuk-Ching Tse-Dinh, Keir C. Neuman Platform Microtubules and Associated Motors 8:15 am–10:15 am, Esplanade, Room 156 Co-Chairs Jing Xu, University of California, Merced Anita Jannasch, Technische Universität Dresden, Germany 980-Plat 8:15 am CRYO-ELECTRON TOMOGRAPHY REVEALS THAT DYNACTIN RECRUITS A TEAM OF DYNEINS FOR PROCESSIVE MOTILITY.  Danielle A. Grotjahn , Saikat Chowdhury, Yiru Xu, McKenney J. Richard, Trina Schroer, Gabriel C. Lander

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