Biophysical Society 63rd Annual Meeting | Program Guide

excitation wavelengths and all emission wavelengths. The matrix is then corrected for effects of high concentration (inner-filter effect) using the absorbance spectrum. The resulting A-TEEM gives an accurate profile of all emitting species and in turn, gives more information about the content of the sample in question, thus making it a better data set for chemomet- ric and quantitative analysis. Solutions of tryptophan and 2-aminopurine, a fluorescent derivative of adenine, are used to demonstrate 1.) Effects of high absorbance/concentration on the fluorescence profile; and 2.) The A-TEEM profile for detection of multiple components. Speaker Karen Gall, Applications Scientist, HORIBA Scientific Career Development Center Workshop Green Cards for Scientific Researchers: How to Brian Getson is a leading U.S. immigration lawyer who represents scien- tific researchers in applying for green cards in the EB-1A, EB-1B and NIW categories. Learn about the U.S. immigration process and how to maxi- mize your chances of immigration success during this workshop. He will answer questions and provide free legal consultations after the presenta- tion and throughout BPS 2019 in the Career Development Center. Symposium Proteins: Exploring Sequence Space via Computation and Experiment 10:45 am - 12:45 pm, Ballroom I Chair Polly Fordyce, Stanford University 117-Symp 10:45 am ENGINEERING AND EVOLUTION OF ALLOSTERIC COMMUNICATION.  Kimberly A. Reynolds No Abstract 11:15 am HOW DO PROTEINS EVOLVE.  Daniel Tawfik 118-Symp 11:45 am HYPERVARIABLE PROTEINS IN MICROBES.  Eugene Koonin 119-Symp 12:15 pm BRINGING ENZYMOLOGY INTO THE GENOMIC ERA: DEVELOPING AND DEPLOYING NEW TOOLS TO QUANTITATIVELY MAP FUNCTIONAL CON- NECTIONS THROUGHOUT AN ENZYME.  Craig Markin, Daniel Mokhtari, Fanny Sunden, Dan Herschlag, Polly M. Fordyce Symposium Glutamate Receptors 10:45 am - 12:45 pm, Ballroom II Chair Maria Kurnikova, Carnegie Mellon University 120-Symp 10:45 am OPTICAL CONTROL AND REPORT OF amPA RECEPTOR ACTIVATION.  Andrew Plested No Abstract 11:15 am ALLOSTERIC DYNAMICS AND DRUGGABILITY OF amPA RECEPTORS.  Win Your EB-1A/NIW Case! with Getson & Schatz, PC 10:30 am - 11:30 am, Exhibit Hall A

When imaging with the Tilt, cells can be kept alive for hours and even days. This is aided by an optional incubation chamber for the Tilt, which allows for precise control of temperature (heating and cooling available), CO2 and humidity. The Tilt light-sheet imaging system is the ideal solution for long-term live- cell imaging of a wide array of samples with the added benefit of being a simple, low cost add-on to an existing inverted microscope. Speaker Chris Baumann, Sales and Product Manager, Mizar Imaging Exhibits 10:00 am - 5:00 pm, Exhibit Hall Coffee Break 10:15 am - 11:00 am, Exhibit Hall Exhibitor Presentation HORIBA Scientific 10:30 am - 12:00 pm, Room 301 UNIQUE FLUORESCENCE MOLECULAR FINGERPRINTING IN ACTION: WHAT CAN CCD DETECTION DO FOR YOU? Fluorescence is a standard tool for the study of changes on the molecular level, but it is now also becoming an emerging technique for molecular fingerprinting and spectral kinetics. The Duetta™ 2-in-1 fluorescence and absorbance spectrometer from HORIBA Scientific is a unique and power- ful benchtop instrument that provides so much more than standard PMT-based scanning benchtop fluorometers. CCD detection technology, and incorporated absorbance measurements, provide more data, with more accuracy, and in less time. In this presentation, HORIBA Scientific will demonstrate two of many methods for which Duetta is uniquely equipped to measure fluorescent samples. First, Duetta can measure protein binding and FRET over the full emission range (250-1100 nm), demonstrating the effects of both donor and acceptor spectra over time with true spectral kinetics. In addition, the method of measur- ing Absorbance-Transmittance Excitation Emission Matrices (A-TEEMs) gives information about the molecular fingerprint of a mixture for use in component analysis of mixtures. The use of the absorbance detector enables inner-filter effect correction, which can easily be overlooked us- ing standard fluorometers. Full Spectral Kinetics and FRET Because Duetta uses a CCD detector for emission detection, kinetics over the entire emission spectrum (250-1100 nm) instead of only at one or two different emission wavelengths. We will demonstrate the binding of a small molecule, 1,8-anilinonaphthalene sulfonate (ANS), to bovine serum albumin protein (BSA) that shows both the decrease in donor emission (BSA) and the increase of the acceptor emission (ANS) as an example of FRET kinetics. The binding of ANS to hydrophobic pockets in BSA is a known phenomenon, but is typically only measured as a kinet- ics experiment at the ANS emission wavelength of 475 nm. Historically, concentration-dependent experiments where emission spectra are col- lected over a range of ANS or protein concentrations, or both, are used to show binding kinetics or FRET as well. Duetta easily measures both the donor BSA (tryptophan) emission as well as the acceptor ANS emission during binding and shows that energy transfer occurs over the full spec- tral range. This is a unique capability for a benchtop fluorometer in the The use of fluorescence for molecular fingerprinting is a relatively new concept and just as exciting if not more so than spectral kinetics. In most applications, changes in fluorescence intensity, or wavelength, or both, correlate to changes in physical properties of a sample. A-TEEM is a method of measuring the full fluorescence contour plot of a sample at all field of biological fluorescence. A-TEEMMolecular Fingerprinting

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Ivet Bahar 121-Symp

11:45 am THE EUKARYOTIC SPECIFIC M4 SEGMENTS ARE ALLOSTERIC CONDUITS FOR NMDA RECEPTOR SIGNALING.  Lonnie Wollmuth

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