Biophysical Society 63rd Annual Meeting | Program Guide

Career Development Center Workshop Networking for Nerds: How to Create Your Dream Career 11:30 am - 12:30 pm, Exhibit Hall A Wanna land your dream job? Get ready to network! Most jobs and other game-changing career opportunities are not advertised, and even if they are, there is usually a short-list of candidates already in mind. So how do you find out about and access the 90% of jobs and other opportunities that are “hidden”? In this workshop, we will focus on proven networking strategies and tactics to identify new opportunities, locate decision-mak- ers within organizations, solidify your reputation and brand in the minds of those who hire, and gain access to hidden jobs and game-changing opportunities. Discover how networking and self-promotion can enable you to land or even create your dream job from scratch! Exhibitor Presentation Asylum Research 11:30 am - 1:00 pm, Room 303 CAPTURING BIOCHEMICAL REACTIONS WITH VIDEO-RATE AFM Oxford Instruments Asylum Research will present the latest data acquired with its Cypher VRS, the world’s first and only full-featured video-rate AFM. The Cypher VRS Atomic Force Microscope sets a new standard with easy operation—enabling high resolution imaging of dynamic events at high speeds, up to 625 lines/second which corresponds to about 10 frames per second. This speed is about 300x faster than typical AFMs and 10x faster than current “fast scanning” AFMs. One of the strengths of traditional AFMs is its capability to monitor dynamic events in near-native conditions (i.e. in liquid at biologically relevant temperatures). However, capturing biological processes in real- time has been challenging up until now. Video rate AFMs provide that temporal resolution, allowing researchers to observe the progression of these reactions and capture kinetics. Video rate AFMs have allowed researchers to conduct a new set of experiments including biochemical reactions, membrane dynamics, conformational changes, self-assembly and degradation. In most cases, the spatial resolution is not compro- mised enabling researchers to locate the target or active site while track- ing the progression of the reaction. They can observe structural dynamics of biomolecules and then correlate it to their function. We will present a set of data to illustrate the potential of this new capability. Examples include DNA digestion and cleavage, DNA origami conformation changes, protein fiber assembly, membrane dynamics in- cluding molecular structure and rearrangement in the bacteriorhodopsin membrane, lipid bilayer growth, assembly of Type I collagen into fibrils and dynamic motion of CTAB hemi-micelles at the solid (HOPG) – liquid (aqueous buffer) interface. Speaker Sophia Hohlbauch, Applications Scientist, Asylum Research The Nuts and Bolts of Preparing Your NSF Grant 12:30 pm - 2:00 pm, Room 321/322/323 The National Science Foundation’s Biological Sciences Directorate strongly supports biophysics researchers through its Division of Molecular and Cellular Biosciences. The division has awarded over $160 million in funding to researchers in 41 states. At this session, program directors and officers with expertise in biophysics will be providing details on the NSF grant-making process as it stands in 2019, with a particular emphasis on grant writing and submission for new and early career investigators. Speaker Engin Serpersu, Program Director, Division of Molecular and Cellular Biosciences, NSF

Exhibitor Presentation Nanion Technologies 12:30 pm - 2:00 pm, Room 301 ION CHANNELS AND TRANSPORTERS IN THE SPOTLIGHT

M O N D A Y

Nanion Technologies is the leading solution provider for electrophysi- ologists since 2002. If you are studying ion channels and electrogenic transporters, our chip- and plate-based devices are well suited to advance your research and screening projects. In our portfolio, you will find instrumentation for automated patch clamp, bilayer recordings, SSM- based electrophysiology, impedance and extracellular field recordings, covering the needs for low, medium and high throughput assays. Our workshop will start with an introduction by Dr. Niels Fertig (CEO, Nanion) and Dr. Andrea Brüggemann (CSO, Nanion), as a guide through the overall capabilities of Nanion´s technology portfolio. In continuation, we will wel- come our speakers, Dr. Jean-Francois Rolland (Axxam) and Prof. Dr. Randy Stockbridge (University of Michigan), among others. As a part of our workshop, Dr. Rolland will focus on his recent work on assay development in ion channel drug discovery, using the high throughput automated patch clamp screening platform, the SyncroPatch 384/768PE. Application areas of this powerful system, recording from up to 768 cells simultaneously, range from high throughput screening (HTS), cardiac safety assessment and efficacy screening, to the analysis of ion channel mutations. The SyncroPatch 384/768PE supports voltage- and current clamp recordings, temperature control, and minimal cell usage. In addition to the use of stably transfected cell lines, more challenging cell assays including stem cell-derived cells, transiently transfected cells or primary cells can be used successfully. In this presentation Dr. Rolland will also discuss the highly promising approach of using optogenetics combined with automated patch clamp technology in HTS. This method, using light to modulate molecular events in a targeted manner in living cells, could lead to cheaper, faster and highly reliable assays, suitable for running the early steps of ion channels’ drug discovery programs, espe- cially when combined to automated electrophysiology. Among others, data obtained from Axxam´s bPAC-HCN2 cell line that was successfully assayed on SyncroPatch 384PE, will be presented. In continuation, Dr. Stockbridge will be focused on electrogenic trans- porter assay technology, the SURFE2R. The SURFE2R N1 (single channel) and SURFE2R 96SE (96 channels) technologies enable label-free real time measurements of electrogenic transporter protein activity. Employing SSM (solid supported membrane)-based electrophysiology, the SURFE2R instruments compensate for the low turnover rate of these proteins by measurement of up to 109 transporters in parallel. Dr. Stockbridge, as an expert in measuring membrane transport function, will present her recent data obtained on the SURFE2R N1 instrument. She has undertaken a comparative mechanistic analysis to understand how drug export func- tion evolved in the SMR (small multidrug resistance) exporters family. This involved screening panels of potential substrates (drugs and other compounds) to understand how substrate specificity differs among the drug exporters, guanidinium exporters, and various evolutionary interme- diates. The Nanion team is excited to meet you at our workshop. Join us to learn more about how our “smart tools for electrophysiologists” can help take your research to the next level! Speakers Andrea Brüggemann, CSO, Nanion Technologies Niels Fertig, CEO, Nanion Technologies Jean-Francois Rolland, Head of Electrophysiology, Axxam Randy Stockbridge, Assistant Professor, University of Michigan

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