Biophysical Society 63rd Annual Meeting | Program Guide

tions lack both structural and functional phenotypes seen in the body, and that the lack of extracellular cues contributes significantly to the disconnect between in vitro experimental results and in vivo observa- tion. We will demonstrate that ECM-inspired substrate nanotopography drastically improves the structural and functional development of a va- riety of cell types. Specifically, we show how NanoSurface Cultureware and the NanoSurface Cytostretcher can be utilized to study the effects of cell-nanotopography interactions on adhesion, signaling, polarity, migration, physiology, and differentiation across many cell types and model systems including cancer biology, human epithelia, and cardio- vascular function. Further, we will describe how the differentiation of induced pluripotent stem cells can be accelerated and enhanced by pro- viding a more biomimetic culture environment. We will also illustrate how the combination of nanotopography and mechanical stretch can enhance the in vitro phenotypes of cells in culture. Speaker Nicholas Geisse, Chief Science Officer, NanoSurface Biomedical Membership Committee Meeting 3:30 pm - 5:30 pm, Room 333 Career Development Center Workshop Developing Your 30-Second Value Statement I have a brand and you have a brand. A brand is simply a promise of value and every successful professional and company is successful in part because they know how to articulate their brand. The ability to communicate your promise of value is vitally important for not only crafting your own career path, but also for finding out about hidden opportunities and jobs. In this workshop, we learn the fundamentals of branding as it relates to career development and planning strategy. We will work together to develop your own 30-second brand statement which you can use in networking, and informational and job interviews. We will discuss the connection between brand, attitude and reputation, and why every interaction with someone affects how people perceive your brand. You will leave this presentation with the ability to elucidate your own brand to whomever you meet, giving you a critical competi- tive edge in your career and the job market. Symposium Chromatin Organization and Regulation: From Physical Principles to Biological Phenomena 4:00 pm - 6:00 pm, Ballroom I Chair Karolin Luger, University of Colorado Boulder 845-Symp 4:00 pm DNA SHAPE SHIFTING AS A GENE THERAPY TOOL.  Jonathan M. Fogg, Qian Wang, Allison Judge, Erik Stricker, B. Montgomery Pettitt, Lynn Zechiedrich 846-Symp 4:30 pm CHROMOSOME ORGANIZATION BY LOOP EXTRUSION AND PHASE SEPA- RATION.  Leonid Mirny 847-Symp 5:00 pm HOW TO READ AND WRITE MECHANICAL INFORMATION IN DNA MOL- ECULES.  Helmut Schiessel No Abstract 5:30 pm OFF TO THE RACES - QUANTITATING THE RECRUITMENT OF PROTEINS (aka Your Elevator Pitch) 4:00 pm - 5:00 pm, Exhibit Hall A

Designing and Implementing Strategies to Prevent and Recover from Burnout 2:30 pm - 4:00 pm, Room 321/322/323 The demands of research can lead to academic burnout at any career stage, significantly harming both our personal and professional lives. Given the challenges facing scientists in the lab, office, and at home, feelings of dissatisfaction, anxiety, exhaustion, and unproductivity can be difficult to avoid. Exiting and recovering from the burnout cycle can be even more challenging. In this interactive workshop, we will discuss concrete strategies to recognize, prevent, and counteract burnout. The goals of these strategies are to manage stress, promote a sense of well- being, improve efficiency, and to help participants revive their genuine enthusiasm for science. Panelists Vasanthi Jayaraman, University of Texas Health Science Center Kenton Swartz, NINDS, NIH Eleonora Zakharian, University of Illinois Exhibitor Presentation Alvéole 2:30 pm - 4:00 pm, Room 301 BIOENGINEERING RELEVANT CELLULAR MICROENVIRONMENTS WITH PRIMO® In vivo, the cellular microenvironment has a crucial impact on the regulation of cell behavior and functions, such as cellular differentia- tion, proliferation and migration. One of the challenges confronting cell biologists is to mimic this microenvironment in vitro in order to more efficiently study living cells and model diseases. To this end, we present the PRIMO device developed by ALVEOLE. This contactless and maskless UV projection system based on the LIMAP technology(1) allows to control the biochemical and mechanical properties of in vitro microenvironments. We will first show that PRIMO is a suitable tool to print biomolecules on substrates (including glass, plastic, soft/ stiff substrates, textured surfaces, etc.) with an exquisite control over protein densities (micropatterning). Then, we will also present how the projected UV light can be used in order to structure photosensitive resists (such as SU8) and create molds onto which elastomeric solutions can be polymerized (microfabrication). Finally, one of our users will share his research conducted with PRIMO. He used this technology in order to structure and functionalize hydrogels (microstructuration combined with micropatterning) paving the way for 3D cell culture onto controlled, reproducible soft substrates(2). Visit www.alveolelab.com for more information. Speakers Aurélien Pasturel, University of Bordeaux, CNRS, Alvéole Pierre-Olivier Strale, Senior Scientist, Alvéole Exhibitor Presentation NanoSurface Biomedical 3:30 pm - 5:00 pm, Room 303 BIOMIMETIC CELL CULTURE PLATFORMS FOR ENHANCING CELL BIOLOGY STUDIES Cells use structural and mechanical cues from the extracellular matrix (ECM) to regulate a broad spectrum of processes such as cell signaling, electrophysiology, differentiation, division, and even life and death. Over the past few decades, the literature has demonstrated that many cell types cultured in conventional flat, rigid, and static culture condi-

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