Biophysical Society 65th Annual Meeting Program Guide

Platform Biophysics Education 10:00 am - 11:30 am

Break 11:30 am - 12:00 pm Poster Presentations and Late Posters 12:00 pm - 1:30 pm General Networking 1:00 pm-2:00 am

F R I D A Y

T U

Chair Samuel Safran, Weizmann Institute of Science, Israel 62-Plat 10:00 am INTRODUCTORY MODELS OF THE COVID-19 PANDEMIC IN THE UNITED STATES. Peter H. Nelson 63-Plat 10:18 am DIGITAL EDUCATIONAL RESOURCES IN SCIENCES FOR VISUAL IMPAIRED STUDENTS IN TIMES OF COVID - 19. Yuly E. Sánchez , Angie V. Rodriguez 64-Plat 10:36 am STRATEGIES FOR ENHANCING REMOTE BIOPHYSICAL EDUCATION: DE- VELOPMENT OF A MODULE BASED TRAINING SERIES. Amanda K. Sharp , Anne M. Brown 65-Plat 10:54 am CLINICAL APPLICATIONS OF BIOPHYSICS-CLINICAL BIOPHYSICS. Semire Uzun Göçmen 66-Plat 11:12 am THE NATIONAL CENTER FOR CRYOEM ACCESS AND TRAINING: NATION- WIDE ACCESS TO CRYOEM TECHNOLOGY AND CURRICULA. Edward T. Eng , Elina Kopylov, Cathleen Castello, Clinton S. Potter, Bridget Carragher Exhibits 10:00 am - 5:00 pm Exhibitor Presentation Mad City Labs Inc 11:30 am - 12:00 pm From iSCAT to SCATTIRSTORM: Adventures in Single-Molecule Microscopy I will describe the design and application of two multi-modal microscopes built around the Mad City Labs RM21 ® single molecule microscope. The first system combines Interferometric Scattering (iSCAT) microscopy, developed by Philipp Kukura’s lab, together with Total Internal Reflec- tion Dark-Field (TIRDM). By labeling kinesin-1 motors on one head with a 30-nm gold nanoparticle, we were able to track motor stepping at 1 kHz temporal resolution and 1-2 nm spatial precision. This allowed us to clarify previously uncovered features of kinesin stepping. In collaboration with Luke Rice, we extended this system to understanding microtubule dynamics by labeling tubulin dimers with 20-nm gold nanoparticles and observing these tubulin reversibly binding at the plus-ends of growing microtubules. These tools have the potential to be applied broadly to single-molecule studies to increase the temporal resolution beyond what is traditionally accessible by fluorescence, while still maintaining the spa- tial resolution provided by point-spread function fitting. In the second part of the talk, I will describe our progress on a multi-modal microscope that we are using for a DOE-funded project to study the mechanism of cellulose degradation by cellulases for bioenergy applications. In par- ticular, we have optimized the ability to combine Interference Reflection Microscopy (IRM) to image cellulose micro- and nanofibers with TIRF to simultaneously image fluorescently-labeled cellulase enzymes degrading the cellulose. Speaker William Hancock, Department of Biomedical Engineering, Pennsylvania State University

E S

Catch up with colleagues and friends in the biophysical community during our networking hour. This event, which is open to all meeting attendees, provides meet-and-greet opportunities to enhance your virtual meeting experience. Exhibitor Presentation Bruker 1:30 pm - 2:00 pm Introduction to Super-Resolution Multiplexed Imaging Applications Using the Vutara VXL Platform For Single-Molecule and Widefield Imaging This talk will introduce the next generation of the Vutara imaging platform, the VXL. While the VXL has been designed and optimized for single mol- ecule localization microscopy, most current methods for super resolution microscopy are limited due to the limited number of dyes compatible with single molecule based super resolution techniques. This talk pres- ents methods for imaging a series of consecutive targets within a sample using single molecule localization microscopy integrated with a software- controlled automated microfluidics system for probe multiplexing. Probe multiplexing allows for the imaging of more than four different targets within a cell. During this talk we will show examples using oligoSTORM and DNA-PAINT methods. OligoSTORM allows for the direct tracing of chromosomes within cells. We will show the three-dimensional trajectory of a multiplexed oligoPAINT labeled chromosome in individual human fibroblast cells using the Vutara platform. We will also show DNA-PAINT based single molecule localization data for antibody labeled targets in cell culture. We will also show how the Vutara can be used for ORCA, a widefield imaging technique that has been developed for high through- put sequential labelling of chromosome targets for generating 3D image data and single cell chromosome conformation maps (similar to Hi-C type data). The VXL with integrated fluidics and SRX software provides a power- ful suite of tools for simultaneous imaging, localization, visualization and statistical analysis of multiplexed data. Speaker Robert Hobson, Applications Scientist, Bruker Break 1:30 pm - 2:00 pm Live Q&A Careers in Industry 2:00 pm - 3:30 pm Join us for a Q&A discussion about scientific careers in industry. Industry panelists will answer audience questions and share their career experi- ences. Hear from professionals and get tangible advice for identifying and pursuing a career in industry. Chair Erin Dueber, Genentech, USA Speakers Muneera Beach, Malvern Panalytical, USA Jeff Hirsch, Confluence Discovery Technologies, USA

D A Y

Aysegul Ozen, Scorpion Therapeutics, USA Jeremy Wilbur, Relay Therapeutics, USA

12