Biophysical Society 66th Annual Meeting Program Guide

12:30 PM – 2:00 PM Nanion Technologies 20 years At the Heart and Mind of Automated Electrophysiology

In our presentation, we will shortly introduce the key distinctive features of our technology and show examples and results obtained by our cus- tomers and scientific collaborators for the aforementioned applications in living cells. Speaker Oriol Nos, CEO, IMPETUX 12:30 PM – 2:00 PM Fluxion Biosciences From Immunology to Ion Channels: Microfluidic Approaches to Automated Patch Clamp and Cellular Analysis. A Look At IonFlux Mercury and BioFlux Systems from Fluxion Biosciences. Leveraging proprietary microfluidic approaches, Fluxion Biosciences provides unique solutions that simplify and automate complex cell- based assays. This presentation will cover two Fluxion systems used extensively in biophysical characterization of cells: IonFlux and BioFlux. IonFlux Mercury automated patch clamp (APC) systems are capable of recording from 16 to 256 separate cells simultaneously in whole cell patch clamp mode. With unique features such as industry-leading fast in-plate compound exchange and continuous solution flow, IonFlux sys- tems are used globally in pharma and academic laboratories for both ligand and voltage-gated ion channel screening. Recent developments include automated IC/EC50 calculation workflows, and the introduction of a new range of GABA cells for complete sample-to-result analysis. The BioFlux system is the world’s leading platform for analyzing cell- cell interactions in a flow-controlled environment. Applications include characterization of cell migration, adhesion strength, transmigration, and chemotaxis. BioFlux assays for research and drug development in immunology, vascular biology, and cancer will be reviewed. Recent research in covid-induced thrombocytopenia and functional analysis of CAR-T cells will be highlighted. Speaker s Jeff Jensen, Chief Executive Officer, Fluxion Biosciences Ali Yehia, Chief Scientific Officer, Fluxion Biosciences 2:30 PM – 4:00 PM Delmic PRESENTATION TITLE AND SUMMARY INCLUDED IN ADDENDUM Esplanade, Room 158: Monday, February 21 10:30 AM – 12:00 PM Bruker PRESENTATION TITLE AND SUMMARY INCLUDED IN ADDENDUM

For 20 years, Nanion Technologies provides diverse solutions for electro- physiologists worldwide. We aim to successfully implement innovative technologies in the fields of ion channel automated patch clamp (APC) electrophysiology, monitoring of cell viability and contraction, as well as electrogenic transporters, with various throughput capabilities. This year, our symposiumwill start with an introduction by Dr. Andrea Brueggemann (CSO, Nanion) who will guide you through the overall capabilities of Nanion´s portfolio. Following this, we will welcome our speakers, whose work focuses on neural and cardiac physiology and pathophysiology. Dr. Elena Govorunova will introduce the application of high throughput APC systems for research in channelrhodopsins (ChRs) used for activity modulation of neurons and cardiomyocytes with light (optogenetics). ChRs exhibit light-gated channel conductance, thereby enabling stimulation and inhibition of neuronal activity due to de- and hyperpolarization of the membrane, respectively. However, electrophysiological characterization of new ChRs lags behind because it is mostly done by time-consuming manual patch clamp. Here, Dr. Govorunova will show how the use of the SyncroPatch 384 benefits this program. Fitzwilliam Seibertz will focus on atrial fibrillation (AF) as the most com- monly reported cardiac arrhythmia. Current AF therapeutics lack efficacy, and mechanistic models to examine ion channel remodeling in AF are lim- ited by a lack of atrial specificity in expression systems or low throughput methodologies. Seibertz will highlight APC technology as a key method to increase throughput in ion channel research, and possibly a crucial tool for mechanistic dissection of complex AF-induced ionic remodeling events. His data will show the capabilities of SyncroPatch 384 in characterizing atrial electrophysiology in a novel atrial-specific human induced pluripo- tent stem cell-derived cardiomyocyte (hiPSC-CMs) construct, and in atrial cardiomyocytes directly isolated from native myocardium . Benefits of a physiological cell culture assay with stimulating effect on hiPSC-CM maturation will be the focus of Dr. Bettina Lickiss. To assess preclinical cardiac risk quickly and with high human relevance, the 96-well FLEXcyte technology is employed, containing flexible membranes that serve as a native-like environment for the cells. Gene expression, pheno- type and functional properties were analyzed as well as the effect of long- term cultures. Dr. Lickiss will compare differences to hiPSC-CMs cultured on regular plates highlighting the pro-maturation effect of the technology for evaluating human relevant inotropic effects beyond the current per- spective of preclinical cardiac risk assessment. Lysosomal, mitochondrial and other internal membranes are moving into focus of transport protein research. However, the localization of these proteins complicates or permits the application of established electro- physiological methods. Dr. Maria Barthmes will introduce an electro- physiological approach based on solid-supported membrane technology (SURFE2R N1) to address cardiac and neuronal transport proteins in intra- cellular membranes. Applying this approach in a pilot study, functional current recordings of TMEM175 expressed in lysosomes were generated. Furthermore, mitochondrial inner membranes were isolated from cardiac tissue and several exchangers and proton transporting complexes were successfully investigated using the SSM-based electrophysiology. Speakers Maria Barthmes, Senior Scientist, Nanion Technologies Andrea Brueggemann, CSO, Nanion Technologies Elena Govorunova, Associate Professor, Center for Membrane Biology, Department of Biochemistry & Molecular Biology, The University of Texas Health Science Center at Houston McGovern Medical School - Houston Bettina Lickiss, innoVitro GmbH, Juelich Fitzwilliam Seibertz, Scientist, University of Göttingen

160