Biophysical Society 66th Annual Meeting Program Guide

2:30 PM – 4:00 PM Sutter Instrument Scientists Empowering Scientists

4:30 PM – 6:00 PM Interherence GmbH

Reproducibility Through Precision: Chip-Based Technologies For Precise Control of Sample Temperature and Illumination in High- and Superresolution Microscopy Biological systems are complex and many environmental parameters affect their behavior. The chemical conditions of the sample vol- ume, including pH and salt concentration, are often well considered when designing experiments in high and super-resolution microscopy. However, control over the physical conditions such as temperature or the amount of light deposited in the sample volume are often not well characterized and might differ from setup to setup. The imprecisions mainly arise due to the lack of appropriate sensor that can be placed close to or directly in the sample volume. Integrated systems, which are in direct contact with the sample, can help to improve reproducibility in biophysical studies of living cells, proteins or DNA and make interpreta- tion of the acquired data easier. Measuring and controlling the temperature of small sample volumes, as is often the case in high and super resolution microscopy, is technologi- cal challenging. In the first part of this talk, we will review state of the art approaches for temperature management in light microscopy, analyze their strengths and weaknesses and talk about our solution, VAHEAT. We will highlight the importance of controlling the temperature in bio- physical studies based on recent research covering the fields of single molecules studies, live cell imaging and colloid chemistry. In the second part of the talk, we will focus on illumination conditions specifically in total internal reflection (TIR) microscopy. After analyzing the limits of current technologies, we take the opportunity to pre-launch QuScite - our device for TIRF microscopy on a chip. This waveguide TIR system relies on photonic integrated circuitry (PIC) and allows to separate the excitation from detection path, which opens up completely new pathways for studying single molecules and processes in cell mem- branes. QuScite’s unprecedented field of view in the range of square millimeters combined with its ease of use even in complex workflows should allow everyone, on any microscope to perform high sensitivity TIRF measurements under reproducible conditions. Note: This presentation will be interactive. You will have the opportunity to see and test demo systems on site. Speakers Jaroslav Icha, CMO, Interherence GmbH Pierre Türschmann, CEO, Interherence GmbH

For over 47 years, Sutter Instrument has been constantly designing equipment so that scientists can push the limits. With an extensive suite of products that include micropipette fabrication, micromanipula- tion, imaging, microscopes, noise isolation, perfusion, and amplifier systems Sutter can be a single source for building a patch clamp rig from the ground up. In our popular series of free user meetings and webinars with tutorial presentations, we provide step-by-step guidance to the new researcher as well as advanced tips and tricks for the experienced users. The focus for this user meeting will be on micropipette fabrication for electro- physiology and microinjection applications, as well as the Sutter ampli- fiers used for patch clamp recording. We will describe research pro- tocols requiring Sutter pipette pullers, the gold standard for creating highly accurate and reproducible pipette geometries, several unique micropipette shapes for specialty applications and use case scenarios for the Sutter amplifier systems. The Amplifier System product line has been expanded and perfec- tioned since its first introduction in 2016. Built on the common theme of integrating the common principle of integrated data acquisition systems and included SutterPatch® Software, the spectrum now spans from the basic IPA® whole-cell system with a single headstage to the flagship dPatch® Amplifier System suitable for sophisticated dynamic clamp experiments, single-channel recordings, and high-bandwidth applications. The digital architecture of the dPatch System constitutes a technology shift that enables dynamic clamp capability without the need for pricey and/or cumbersome external components. In the Q&A session, the speakers will also be available for questions or detailed suggestions on particular applications. Who should attend? • Electrophysiologists who use amplifiers, micropipettes and micromanipulators for patch clamp, sharp electrode, or extracellular recordings. • Researchers who perform patch clamp recordings or micro- injections in tissue slices, dissociated cells, cell lines or in vivo preparations. • Anybody who is interested in pulling micropipettes, or in the latest feature-rich hardware and software for electrophysiol- ogy applications. Speakers Jan Dolzer, Director of Marketing, Sutter Instrument Telly Galiatsatos, Tech Support and Product Development, Sutter Instrument Adair Oesterle, Support Engineer, Sutter Instrument

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