Biophysical Society 66th Annual Meeting Program Guide

Exhibitor Presentation Nanion Technologies 12:30 pm - 2:00 pm, Esplanade, Room 158 20 Years At the Heart and Mind of Automated Electrophysiology For 20 years, Nanion Technologies provides diverse solutions for electro - physiologists worldwide. We aim to successfully implement innovative technologies in the fields of ion channel automated patch clamp (APC) electrophysiology, monitoring of cell viability and contraction, as well as electrogenic transporters, with various throughput capabilities. This year, our symposium will start with an introduction by Dr. Andrea Bruegge - mann (CSO, Nanion) who will guide you through the overall capabilities of Nanion´s portfolio. Following this, we will welcome our speakers, whose work focuses on neural and cardiac physiology and pathophysiology. Dr. Elena Govorunova will introduce the application of high throughput APC systems for research in channelrhodopsins (ChRs) used for activity modulation of neurons and cardiomyocytes with light (optogenetics). ChRs exhibit light-gated channel conductance, thereby enabling stimula - tion and inhibition of neuronal activity due to de- and hyperpolarization of the membrane, respectively. However, electrophysiological charac - terization of new ChRs lags behind because it is mostly done by time- consuming manual patch clamp. Here, Dr. Govorunova will show how the use of the SyncroPatch 384 benefits this program. Fitzwilliam Seibertz will focus on atrial fibrillation (AF) as the most com - monly reported cardiac arrhythmia. Current AF therapeutics lack efficacy, and mechanistic models to examine ion channel remodeling in AF are limited by a lack of atrial specificity in expression systems or low through - put methodologies. Seibertz will highlight APC technology as a key meth - od to increase throughput in ion channel research, and possibly a crucial tool for mechanistic dissection of complex AF-induced ionic remodeling events. His data will show the capabilities of SyncroPatch 384 in charac - terizing atrial electrophysiology in a novel atrial-specific human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CMs) construct, and in atrial cardiomyocytes directly isolated from native myocardium. Benefits of a physiological cell culture assay with stimulating effect on hiPSC-CMmaturation will be the focus of Dr. Bettina Lickiss. To assess preclinical cardiac risk quickly and with high human relevance, the 96- well FLEXcyte technology is employed, containing flexible membranes that serve as a native-like environment for the cells. Gene expression, phenotype and functional properties were analyzed as well as the effect of long-term cultures. Dr. Lickiss will compare differences to hiPSC-CMs cultured on regular plates highlighting the pro-maturation effect of the technology for evaluating human relevant inotropic effects beyond the current perspective of preclinical cardiac risk assessment. Lysosomal, mitochondrial and other internal membranes are moving into focus of transport protein research. However, the localization of these proteins complicates or permits the application of established electro - physiological methods. Dr. Maria Barthmes will introduce an electro - physiological approach based on solid-supported membrane technology (SURFE2R N1) to address cardiac and neuronal transport proteins in in - tracellular membranes. Applying this approach in a pilot study, functional current recordings of TMEM175 expressed in lysosomes were generated. Furthermore, mitochondrial inner membranes were isolated from cardiac tissue and several exchangers and proton transporting complexes were successfully investigated using the SSM-based electrophysiology. Speakers Maria Barthmes, Senior Scientist, Nanion Technologies Andrea Brueggemann, CSO, Nanion Technologies Elena Govorunova, Associate Professor, Center for Membrane Biology, Department of Biochemistry & Molecular Biology, The University of Texas Health Science Center at Houston McGovern Medical School - Houston Bettina Lickiss, innoVitro GmbH, Juelich Fitzwilliam Seibertz, Scientist, University of Göttingen

Annual Meeting of the Student Chapters 11:00 am - 1:00 pm, Esplanade, Room 156 Join BPS Student Chapters from all over the world for a poster ses - sion and workshop. Attendees will meet Student Chapter officers and representatives and learn about each chapter. The interactive workshop aims to establish chapter interactions, communications, and planning for future Student Chapter Annual Meeting sessions. Exhibitor Presentation LEICA MICROSYSTEMS INC 11:30 am - 1:00 pm, Esplanade, Room 157 Label-Free, Chemically Specific Imaging with the Leica STELLARIS 8 CRS Coherent Raman Scattering microscopy (CRS) is a powerful new imaging technique that provides label-free, chemically specific image contrast based on the characteristic intrinsic vibrational of the sample molecules. CRS can produce high-resolution (sub-cellular level), dynamic (up to video-rate), and quantifiable information on the biochemical composition and metabolic processes in cells, tissues, and intact model organisms, and it enables imaging of small molecules without perturbing their function. This information is highly synergistic with the types of contrast provided by fluorescence microscopy. Here, I will provide an overview of the wide range of application areas covered by the all-new STELLARIS 8 CRS – Leica’s hands-free Coherent Raman Scattering microscope. The instrument offers both CRS modalities – Stimulated Raman Scattering (SRS) and CARS – and allows for the simul - taneous acquisition of two-photon fluorescence and second-harmonic generation signals. Importantly, the seamless integration of CRS with the STELLARIS visible confocal fluorescence microscopy platform results in a true multi-modal optical discovery instrument that is capable of captur - ing a unique combination of biochemical, biophysical and molecular contrasts. Unsurprisingly, the instrument is finding a growing number of applications in fields like neurodegenerative disease, cancer, 3D biology, stem cell and developmental biology, and pharmacology. Speaker Volker Schweikhard, Global Application Manager, LEICA MICROSYSTEMS INC Career Development Center Workshop Translating Your Credentials: Writing Effective Resumes + Cover Letters for Industry, You have a CV with its list of publications, presentations, honors & awards, etc., but jobs outside of academia typically require a resume. Very few job ads fail to mention the importance of teamwork, collabora - tion, and communication skills, yet if you take a glance at your CV, these are all likely to be absent or incomplete. Learn how to transform your CV into an effective resume that helps hiring managers recognize and ap - preciate the value you have to offer. Government, and Nonprofit Jobs 12:00 pm - 1:00 pm, Esplanade, Room 151

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