Biophysical Society 67th Annual Meeting Program Guide

69-plat 8:45 am CD20-DEPENDENT ACTIN REMODELLING REGULATES INITIAL STEPS OF B CELL ACTIVATION. Anna T. Bajur , Hannah C.W. McArthur, Maro Iliopou lou, Katelyn M. Spillane 70-plat 9:00 am OPTICALLY ACTIVATED PROXIMITY LABELING FOR SPATIALLY AND TEMPO RALLY RESOLVED PROTEOMICS. Omer M. Shafraz, Carolyn Davis, Sanjeevi Sivasankar 71-plat 9:15 am UNNATURAL AMI NO ACID RECEPTOR INCORPORATION AS A PHOTOAF FINITY TOOL FOR GPCR HETEROMER SIGNALING STUDIES. Brenda T. Winn , Roman Manetsch, Diomedes E. Logothetis 72-plat 9:30 am TWO-STEP STRUCTURAL CHANGES IN M3 MUSCARINIC RECEPTOR ACTIVATION RELY ON THE COUPLED G PROTEIN CYCLE. Yong-Seok Kim, Jun-Hee Yeon, Byung C. Suh 73-plat 9:45 am PROTEIN ENGINEERING APPROACHES TO DETERMINE ADENOSINE RECEPTOR-G PROTEIN INTERACTIONS. Anne S. Robinson , Kirsten S. Koretz, Abhinav R. Jain 74-plat 10:00 am MITOCHONDRIAL BOUND PKA CONTRIBUTES TO CYCLIC-AMP COMPART MENTATION IN HUMAN AIRWAY SMOOTH MUSCLE CELLS. Rinzhin Tshering Sherpa , Karni S. Moshal, Shailesh R. Agarwal, Rennolds S. Os trom, Robert D. Harvey Platform Optical Microscopy and Superresolution Imaging I 8:15 am - 10:15 am, Room 11AB Co-Chairs 75-plat 8:15 am EVENT-DRIVEN ACQUISITION FOR CONTENT-ENRICHED MICROSCOPY. Dora Mahecic, Willi L. Stepp , Chen Zhang, Juliette Griffie, Martin Weigert, Suliana Manley 76-plat 8:30 am MULTI-COLOR SUPERRESOLUTION IMAGING TO STUDY HUMAN CORO NAVIRUS RNA DURING CELLULAR INFECTION. Anish R. Roy , Jiarui Wang, Mengting Han, Haifeng Wang, Leonhard Möckl, Leiping Zeng, William E. Moerner, Lei S. Qi 77-plat 8:45 am Travel Award Winner IN-SITU CHARACTERIZATION OF ALZHEIMER’S DISEASE TAU AGGREGATES VIA SUPERRESOLUTION IMAGING. Adriana N. Santiago Ruiz , Arian Arab, Siewert Hugelier, Charles Bond, Melike Lakadamyali 78-plat 9:00 am DIRECT OBSERVATION AND CLASSIFICATION OF HETEROGENEOUS PRO TEIN AGGREGATION IN REAL-TIME THROUGH SUPERRESOLUTION AND AGGREGATIONAL FINGERPRINTING. Steen Bender , Jacob Kæstel-hansen, Min Zhang, Nikos S. Hatzakis 9:15 am Flash Talks Steen Bender, University of Copenhagen, Denmark Eleonora Perego, Istituto Italiano di Tecnologia, Italy

79-plat 9:30 am COMPREHENSIVE FLUORESCENCE LIFETIME FLUCTUATION SPECTROS COPY TO INVESTIGATE PROTEIN MOBILITY DURING AGGREGATION PRO CESSES IN LIVING CELLS. Eleonora Perego , Sabrina Zappone, Eli Slenders, Francesco Castagnetti, Erika Vitiello, Davide Mariani, Lorenzo S. Mecarelli, Irene Bozzoni, Giuseppe Vicidomini 80 -plat 9:45 am SINGLE-MOLECULE LOCALIZATION MICROSCOPY OF EXPANDED MAM MALIAN CENTRIOLES UNRAVELS THE ULTRASTRUCTURAL CONSTITUTION OF DISTAL APPENDAGES. Ting-Jui B. Chang , Jimmy C-C. Hsu, Tony Yang 81-plat 10:00 am Travel Award Winner IDENTIFYING THE ROLE OF THE FACT HISTONE CHAPERONE IN EPI GENETICS THROUGH SINGLE-MOLECULE FLUORESCENCE IMAGING. Yekaterina Fyodorova , Saikat Biswas, Kaushik Ragunathan, Julie S. Biteen CID Committee Meeting 8:30 am - 10:30 am, Room 14A Career Development Center Workshop Networking for Biophysicists: How to Create Your Dream Career 9:00 am - 10:00 am, Room 16B In this session, we will discuss how to leverage networking to create a unique, customized career, which we call Your Dream Career. We will address how to understand your unique value, skills, and interests and how to align that with employers who will hire you. We will discuss vari ous methods for finding and building win-win collaborations with people in different professions and pathways to create opportunities for your dream career to come to fruition. Exhibitor Presentation Oroboros Instruments GmbH 9:30 am - 11:00 am, Room 9 Mitochondrial Bioenergetics – A Quantitative Analytical and Diagnostic Approach Mitochondrial fitness is essential for brain and muscle function, for resistance against preventable and age-related degenerative diseases and therefore for quality of life. The capacity of oxidative phosphorylation (OXPHOS) is a fundamental part of mitochondrial fitness and a key ele ment in bioenergetics. Comprehensive and real-time OXPHOS analysis is based on biophysical and biochemical concepts related to mitochondrial core energy metabolism. It extends bioenergetics to the level of mito chondrial physiology for functional diagnosis in health and disease. The Oroboros O2k is the state-of-the art respirometer for quantitative high-resolution respirometry (HRR) and comprehensive OXPHOS analysis. It has the high signal stability and unrestricted flexibility of titrations suited for application of complex substrate-uncoupler-inhibitor titration (SUIT) protocols which are the basis for investigating mitochondrial path ways and respiratory control in health and disease. High resolution and precise control of oxygen concentration enable studies of mitochondrial functions under normoxia, hypoxia, and hyperoxia. With the O2k-FluoRespirometer, fluorometric assays for ROS production, mitochondrial membrane potential, ATP production, and calcium uptake can be measured in real-time and simultaneously in direct conjunc tion with HRR. Modules for monitoring Q- and NAD-redox states and PhotoBiology are implemented in the NextGen-O2k, further extending the analytical resolution and opening new windows to study biophysical principles of bioenergetics.

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