Program Book

8:15 am –10:15 am , R oom 306 Platform DNA Replication, Recombination, and Repair Co-Chairs Marcia Levitus, Arizona State University Susan Tsutakawa, Lawrence Berkeley National Laboratory 1159-P lat 8:15 am IDENTIFICATION OF THE TRANSLOCATION STEP OF A REPLICATIVE DNA POLYMERASE. Jose A. Morin, Francisco J. Cao, Jose M. Lázaro, Margarita Salas, Jose M. Valpuesta, Jose L. Carrascosa, Borja Ibarra 1160-P lat 8:30 am SINGLE MOLECULE STUDIES OF DNA REPLICATION PROCESSIVITY CLAMPS. Jennifer K. Binder , Suman Ranjit, Manas Chakraborty, David Kanno, Lauren Douma, Linda Bloom, Marcia Levitus 1161-P lat 8:45 am DEFINING THE SUPERFAMILY CONSERVED MECHANISM FOR FLAP ENDONUCLEASES FEN1 AND XPG SPECIFICITY FOR 5’ FLAP DNA AND DNA BUBBLES, RESPECTIVELY, BY HYBRIDS METHODS OF CRYSTALLOGRAPHY, SAXS, EM, AND COMPU- TATION. Susan Tsutakawa , Andrew Arvai, Altaf Sarker, Jordi Querol-Audi, David Finger, Eva Nogales, Ivaylo Ivanov, Priscilla Cooper, Jane Grasby, John Tainer 1162-P lat 9:00 am ROADBLOCKS ON THE E.COLI GENOME: THE WORKINGS OF A MOLECULAR MOUSE TRAP AT THE SINGLE-MOLECULE LEVEL. Bojk A. Berghuis , David Dulin, Bronwen Cross, Nicholas E. Dixon, Nynke H. Dekker 1163-P lat 9:15 am SLIDING, PAUSING AND BRIDGING: HOWHUMAN XRCC4 AND XLF INTERACTWITHDNA. Andrea Candelli, Gerrit Sitters, Ineke Brouwer, Stephanie Heerema, Mauro Modesti, Erwin Peterman, Gijs J.Wuite 1164-P lat 9:30 am SINGLE DNA GLYCOSYLASE MOLECULES DIFFUSE ONE- DIMENSIONALLY ANDUSE AWEDGE RESIDUETO PROBE FOR OXIDATIVELY DAMAGED BASES. Shane Nelson , Andrew Dunn, Susan S. Wallace, David M. Warshaw 1165-P lat 9:45 am ONE ORC WITH MANY FACES. Huilin Li 1166-P lat 10:00 am INTERACTIONS BETWEEN THE E. COLI SOS RESPONSE PROTEIN UMUD AND DNA POLYMERASE III ALPHA SUBUNIT HAVE IMPLICATIONS FOR REGULATING REPLICATION IN RESPONSE TO DNA DAMAGE. Penny Beuning , Michelle C. Silva, Philip Nevin, Kathy R. Chaurasiya, Clarissa Ruslie, Lukas Voortman, Samer Lone, Erin A. Ronayne, Mark C. Williams

in biological systems and processes under physiological conditions; however, certain limitations for AFM still exist in the field of bio-applications. In recent times, the development of another kind of scanning probe microscopy (SPM) technique, scanning ion conductance microscopy (SICM), has overcome these limitations and enabled noninvasive, nanoscale investigation of live cells. SICM applications include imaging of cell topography, monitoring of live cell dynam- ics, mechanical stimulation of live cells, surface patterning, and so forth. We at Park Systems have developed AFM for advanced nanoscale metrology, which separates the z-scanner from the x-y scanner. An independent z-scanner also provides an excellent platform for developing other SPM techniques such as SICM. In addition, the platform which separates the z-scanner from x-y scanner, enables us to easily switch between an AFM and an SICM z-scanner to apply both techniques without moving samples. The common glass micropipette is used in SICM as the sensitive probe, instead of a silicon-based stylus, and can glide over live cells while maintaining an absolute non-contact imaging mode. Its electrochemical current feedback system further enhances biological sample imaging. Combining confocal fluorescence data to the SICM 3D data, using an image overlay feature, provides even more data about structure of cells as related to their membranes. These advances of convergence in instrumentation will be utilized in various kinds of biomedical research and become a new driving force for biophysics and nanobioscience. Presenter: Sangjoon Cho, Sr. Director of R&D, Park Systems, Inc. 10:00 am –11:00 am , R oom 300 Career Center Workshop Career Open Forum/Career Q&A Session Bring your coffee and start your day with this industry-focused forum, in- tended to answer any/all of your questions related to your job search. Space is limited! This is your opportunity to explore the myths and realities associ- ated with an effective job search. Come with any and all job search-related questions and we’ll do our best to provide answers and guidance. 10:00 am –5:00 pm , H all D Biomolecular Discovery Dome 10:00 am –5:00 pm , H all D Exhibits 10:15 am –11:00 am , H all D Coffee Break 10:15 am –11:15 am , R oom 302 New Member Welcome Coffee All new Biophysical Society members are invited to participate in an informal gathering to meet members of the Society’s council and committees, find out about the Society’s activities, get acquainted with other new members, and enjoy refreshments. Current members are encouraged to come meet the newmembers. 10:45 am –12:45 pm , R oom 134 Symposium Biophysics of Personalized Medicine Co-Chairs Donald Engelman, Yale University Kathleen Giacomini, University of California, San Francisco 1167-S ymp 10:45 am IMAGING ANDTREATINGTUMORS BYTARGETINGTHEIR ACIDITYWITH PHLIP, A PH-SENSITVE INSERTION PEPTIDE. DonaldM. Engelman , Ming An, Oleg A. Andreev, Francisco N. Barrera, Raman Bahal, Marcus W. Bosenberg, Christopher Cheng, Peter M. Glazer, Alexander Karabadzhak, Yana K. Reshetnyak, W. Mark Saltzman, Frank J. Slack, Alexander A. Svoronos, DamienThevenin

M O N D A Y

8:30 am –10:00 am , R oom 122 Minority Affairs Committee Meeting

9:00 am –10:30 am , R oom 123 Exhibitor Presentation Park Systems, Inc.

New Door to Live Single Cell Research Atomic Force Microscopy (AFM) is a powerful measurement technique for nanoscale science. AFM is able to provide high-resolution imaging of biological structures below the optical limit, as well as the monitoring of the dynamics

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Biophysical Society 58 th Annual Meeting, San Francisco, California