BPS2025 Full Program

194-FlashTalk 5:45 pm RECOVERING TRUE FRET EFFICIENCIES FROM SMFRET INVESTIGATIONS REQUIRES TRIPLET STATE MITIGATION. Avik K. Pati, Zeliha Kilic , Maxwell I. Martin, Daniel S. Terry, Alessandro Borgia, Sukanta Bar, Steffen Jock usch, Roman Kiselev, Roger B. Altman, Scott C. Blanchard 195-FlashTalk 5:50 pm Travel Award Winner NANOPHOTONIC DYECYCLING BREAKS THE PHOTOBLEACHING LIMIT IN SINGLE-MOLECULE FRET. Benjamin Vermeer , Dong Hoon Shin, Sabina Caneva, Sonja Schmid 196-FlashTalk 5:55 pm EXPANDING THE REACH OF FRET: QUANTITATIVE QUENCHING FOR SUB 3 NM DISTANCE DETERMINATION. Sophie E. Fountain , Victoria E. Hill, Benjamin Ambrose, Joseph White, Robert A. Shaw, Tristan Johnston Wood, Matthew Willmott, Callum Johnston, Rebecca Rebecca Mächtel, Gabriel Rosenblum, Eitan Lerner, Grant Hill, Hagen Hofmann, Thorben Cordes, Timothy D. Craggs Platform Membrane Pumps, Transporters, and Exchangers 4:00 pm - 6:00 pm, Room 501ABC Co-Chairs Jacob Hilton, NIH, USA Doreen Matthies, NICHD, NIH, USA 197-Plat 4:00 pm STRUCTURE OF THE TWIN-ARGININE TRANSLOCATION PATHWAY CORE COMPLEX AND THE MOLECULAR BASIS FOR SUBSTRATE RECOGNITION. Owain J. Bryant , Justin C. Deme, Ben C. Berks, Susan M. Lea 198-Plat 4:15 pm MAGNESIUM TRANSPORTER MGTA REVEALED AS A DIMERIC P-TYPE ATPASE. Rilee Zeinert, Fei Zhou, Pedro Franco, Jonathan Zöller, Zaid K. Madni, Henry J. Lessen, Aravind Iyer, Julian Langer, Alexander J. Sodt, Gisela Storz, Doreen Matthies 199-Plat 4:30 pm GLUTATHIONE TRANSPORT THROUGH THE MALARIA PARASITE’S CHLO ROQUINE RESISTANCE TRANSPORTER, PFCRT. Eva Gil-Iturbe , Jonathan Kim, Laura M. Hagenah, Filippo Mancia, David A. Fidock, Matthias Quick 200-Plat 4:45 pm TRANSPORTER DE-OLIGOMERIZATION REGULATES COPPER UPTAKE INTO HUMAN CELLS. Tai-Yen Chen 201-Plat 5:00 pm CAPTURING THE STRUCTURAL DYNAMICS AND ENERGETICS OF THE LARGE-SCALE ELEVATOR TRANSITION OF NACT. Noah Trebesch , Emad Tajkhorshid 202-Plat 5:15 pm STRUCTURAL DETERMINANTS OF CLC-7 VOLTAGE DEPENDENCE, GAT ING, AND CHLORIDE/PROTON COUPLING. Jacob K. Hilton , Yifei Lin, Justin Deme, Joanne Parker, Michael Grabe, Susan Lea, Simon News tead, Joseph A. Mindell 203-Plat 5:30 pm PHARMACOLOGY OF THE MAMMALIAN BRAIN V-ATPASE STUDIED AT THE SINGLE MOLECULE LEVEL IN INTACT SYNAPTIC VESICLES. Elefthe rios I. Kosmidis, Maria Fokaeos 204-FlashTalk 5:45 pm FROM SEQUENCE TO MECHANISM: PROBING THE ALTERNATING-AC CESS CYCLE OF CYSTINOSIN WITH ENHANCED-SAMPLING MOLECULAR DYNAMICS AND QM/MM SIMULATIONS. Jonathan D. Colburn , Simon Newstead, Philip C. Biggin

205-FlashTalk 5:50 pm STRUCTURAL AND MOLECULAR BASIS OF CHOLINE UPTAKE INTO THE BRAIN BY FLVCR2. Rosemary J. Cater , Dibyanti Mukherjee, Thomas Arnold, Filippo Mancia 206-FlashTalk 5:55 pm THE INHIBITION MECHANISM OF URATE TRANSPORTER URAT1 BY GOUT DRUGS. Justin G. Fedor , Yang Suo, Han Zhang, Kalina Tsolova, Ziaoyu Shi, Kedar Sharma, Shweta Kumari, Mario J. Borgnia, Peng Zhan, Wonpil Im, Seok-Yong Lee Platform Motor Proteins and Cytoskeletons I 4:00 pm - 6:00 pm, Room 511ABC Co-Chairs CONFINEMENT OF ACTIN WITHIN VASP DROPLETS PROMOTES ACTIN RING FORMATION AND DRIVES DROPLET SHAPE CHANGE. Aravind Chandrasekaran , Kristin Graham, Aly Ladak, Liping Wang, Eileen M. Lafer, Jeanne C. Stachowiak, Padmini Rangamani 208-Plat 4:15 pm EXPLORING ISOFORM DEPENDENT ARP2/3 STRUCTURAL FLEXIBILITY THROUGH CRYO-EM. Alana R. Cowell , Ramteen Shayan, Miroslav Mlad enov, Michael Way, Carolyn A. Moores 209-Plat 4:30 pm IMPACT OF MAGNESIUM AND IONIC STRENGTH REVEAL ROLE OF CRUCIAL SALT BRIDGE IN THE MOTOR DOMAIN OF HUMAN CARDIAC MYOSIN ABOLISHED BY THE E525K MUTATION. Jinghua Ge, Michael R. Ebert, Skylar M.L. Bodt, Christopher M. Yengo 210-Plat 4:45 pm Travel Award Winner INTRINSICALLY DISORDERED REGIONS ARE CRUCIAL FOR MYOSIN 10 CARGO BINDING AS REVEALED BY HDX/XL-MS & DNA-PAINT. Julia Shangguan , Allen Huff, Samuel Weng, Susanne Reinhardt, Ralf Jung mann, Ronald S. Rock, Tobin R. Sosnick 211-Plat 5:00 pm CRYO-ET ANALYSIS OF CILIA REVEALS CONFORMATIONAL CHANGE OF DYNEINS AND THEIR COOPERATION WITH OTHER PROTEINS IN SITU. Takashi Ishikawa , Noemi Zimmermann, Leo Luo 212-Plat 5:15 pm UTILIZING THE BACULOVIRUS EXPRESSION SYSTEM TO STUDY KINESIN-2 MOTILITY IN PRIMARY CILIA. Shayna Redford , Rong Liu 213-Plat 5:30 pm STEPPING DYNAMICS OF DYNEIN CHARACTERIZED BY MINFLUX. Joseph Slivka , Emma Gleave, Devinda P. Wijewardena, John Canty, Paul R. Selvin, Andrew Carter, Ahmet Yildiz 214-FlashTalk 5:45 pm AUTOMATED TRACKING OF ACTIN LEARNED STRUCTURES (ATLAS) IN THE MOTILITY ASSAY. Sebastian Duno-Miranda , David M. Warshaw, 5:50 pm MOLECULAR MECHANISM OF ACTIN FILAMENT STIFFENING BY CAT IONS. Xiao-Ping Xu, Wenxiang Cao, Charles V. Sindelar, Enrique M. De La Cruz, Niels Volkmann, Dorit Hanein Shane R. Nelson 215-FlashTalk Takashi Ishikawa, Paul Scherrer Institute, Switzerland Christopher Yengo, Pennsylvania State University, USA 207-Plat 4:00 pm

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