Biophysical Society 66th Annual Meeting Program Guide

Platform Intracellular Dynamics 8:15 am - 10:15 am, South, Level Two, Room 212/213/214 Co-Chairs Erdem Karatekin, Yale University, USA Kate White, University of Southern California, USA 1414-Plat 8:15 am MEMBRANE ORDER REGULATES SNARE MEDIATED VESICLE FUSION IN INSULIN-SECRETING CELLS. Chase Amos, Volker Kiessling , Noah Schenk, Ramkumar Mohan, Catherine A. Doyle, Alex J.B. Kreutzberger, Hongyin Wang, Kandice R. Levental, Ilya Levental, Arun Anantharam, Lukas K. Tamm 1415-Plat 8:30 am MECHANISMS OF DYNAMIN-MEDIATED ULTRAFAST ENDOCYTOSIS. Shigeki Watanabe 1416-Plat 8:45 am POLYBASIC PATCHES IN BOTH C2 DOMAINS OF SYNAPTOTAGMIN-1 ARE REQUIRED FOR EVOKED NEUROTRANSMITTER RELEASE. Zhenyong Wu, Maria Tsemperouli, Lu Ma, Nicholas Courtney, Jie Zhu, Yongli Zhang, Edwin R. Chapman, Erdem Karatekin 1417-Plat 9:00 am CONFORMATIONAL DYNAMICS OF SNARE RECYCLING MEDIATED BY NSF. Katie M. Dunleavy, Holland Matlock, Claire Gething, Giovanni Howells, Bishal Misra, K. Ian White, Axel T. Brunger, Ucheor B. Choi 1418-Plat 9:15 am ENDOTHELIAL CELLS RELEASE HYBRID EXTRACELLULAR VESICLES: MI - CROVESICLES THAT SECRETE EXOSOMES. Jennifer D. Petersen , Elena Mekhedov, Sukhbir Kaur, David D. Roberts, Joshua Zimmerberg 1419-Plat 9:30 am MAPPING THE REGULATORY LANDSCAPE OF MEMBRANELESS ORGANELLE ASSEMBLY AND DYNAMICS. Jessica Sheu-Gruttadauria , Xiaowei Yan, Nico Stuurman, Stephen Floor, Ronald D. Vale 1420-Plat 9:45 am CONTROL OF NUCLEAR SIZE BY OSMOTIC FORCES IN FISSION YEAST. Joël P.C. Lemiere , Paula Real-Calderon, Thomas G. Fai, Fred Chang 1421-Plat 10:00 am USE OF SOFT X-RAY TOMOGRAPHY TO CAPTURE THE DYNAMIC BIOCHEMI - CAL NATURE OF INTACT PANCREATIC BETA CELLS. Kate L. White Platform Voltage-Gated Channels II 8:15 am - 10:15 am, South, Level Two, Room 215/216 Co-Chairs Lisandra Flores Aldama, University of Wisconsin-Madison, USA Crina Nimigean, Weill Medical College, USA 1422-Plat 8:15 am BALL-AND-CHAIN INACTIVATION IN MTHK CHANNEL DEPENDS ON LIPID BILAYER THICKNESS. Nattakan Sukomon , Chen Fan, Alma P. Perrino, Simon Scheuring, Crina M. Nimigean 1423-Plat 8:30 am STRUCTURE OF THE SHAKER KV CHANNEL AND MECHANISM OF SLOW C-TYPE INACTIVATION. Xiaofeng Tan , Chanhyung Bae, Robyn Stix, Ana I. Fernández-Mariño, Kate Huffer, Tsg-Hui Chang, Jiansen Jiang, José D. Faraldo-Gómez, Kenton Swartz 1424-Plat 8:45 am COUPLING BETWEEN CA 2+ BINDING AND THE ACTIVATION GATE OPENING IN BK CHANNELS PROBED BY AN ALLOSTERIC ACTIVATOR. Guohui Zhang , Xianjin Xu, ZhiGuang Jia, Yanyan Geng, Hongwu Liang, Jingyi Shi, Martina Marras, Carlota Abella, Karl L. Magleby, Jonathan R. Silva, Jianhan Chen, Xiaoqin Zou, Jianmin Cui

1425-Plat 9:00 am SIDE CHAIN INTERACTIONS IN C-TERMINAL S4 HELIX REQUIRED FOR GATING POLARITY OF HCN CHANNELS. Xiaoan Wu , Rosamary Ramentol, Marta E. Perez, Peter H. Larsson 1426-Plat 9:15 am CRYO-EM STRUCTURE OF THE MAMMALIAN KV2.1 DELAYED-RECTIFIER POTASSIUM CHANNEL REVEALS INSIGHT INTO GATING MECHANISMS AND EPILEPTIC ENCEPHALOPATHY. Ana I. Fernández-Mariño , Xiaofeng Tan, Chanhyung Bae, Robyn Stix, Tsg-Hui Chang, Jiansen Jiang, José D. Faraldo- Gómez, Kenton Swartz 1427-Plat 9:30 am DISTINCTIVE MECHANISMS OF EPILEPSY-CAUSING MUTANTS DISCOV - ERED BY MEASURING S4 MOVEMENT IN KCNQ2 CHANNELS. Michaela A. Edmond , Andy Hinojo-Perez, Marta E. Perez, Rene Barro-Soria 1428-Plat 9:45 am DIFFERENTIAL HERG 1 SUBUNIT TRANSCRIPTION MEDIATES I KR INHIBITION BY PROTONS IN HUMAN STEM CELL-DERIVED CARDIOMYOCYTES. Chiamaka Ukachukwu , Eric N. Jiménez-Vázquez, Abhilasha Jain, David K. Jones 1429-Plat 10:00 am Travel Award Winner A HYDROPHOBIC NEXUS AT THE PAS-CAP/GLOBULAR PAS/CNBHD IN - TERFACE TUNES DEACTIVATION KINETICS OF HERG CHANNELS. Lisandra Flores Aldama , Whitney A. Stevens-Sostre, Daniel Bustos Guajardo, Gail A. Robertson Subgroup Chairs Meeting 9:00 am - 10:30 am, South, Level Three, Room 313/314 Career Development Center Workshop Looking Beyond Academia: Identifying Your Career Options using MyIDP, LinkedIn & More 9:30 am - 10:30 am, Esplanade, Room 151 Not sure where your professional future lies or how to approach the process in an organized and strategic manner? This presentation provides a framework and resources for moving forward with confidence towards the next step in your professional future. In addition, it will provide specific examples of how to build out your knowledge of a new potential career field and forge valuable connections that can facilitate a successful transition. Use of iPSC-Derived DRG Neurones on Sophion Bioscience Systems. Screening For Sodium Channel and KCa Modulators and Positive Alloste- ric Modulators For GABA Channels. Development of Assays For Novel Targets on the Qube. Successful ion channel drug discovery requires the integration of multiple technologies and workflows. Sophion Bioscience is a leader in automated patch clamp technology, providing low, medium and high throughput patch clamp systems to the drug discovery industry and academia. The QPatch and Qube are fully automated patch clamp systems, executing simultaneous 16, 48 or 384 parallel patch clamp recordings in conjunc - tion with computer controlled liquid handling and on-board cell handling. The QPatch Compact is a manual patch clamp system that can execute individual or simultaneous 8 patch clamp recordings. Sophion provides customers with robust, ion channel and electrophysiological workflows for drug development of ion channel targets. During this workshop, three industry speakers will provide insight into the use of these systems in the drug discovery process. Dr Daniel Sauter will present automated patch clamp data to show use of iPSC-derived neurons on Sophion Bioscience systems. Professor Heike Wulff will present data and discuss screening for Exhibitor Presentation Sophion Bioscience A/S 9:30 am - 11:00 am, Esplanade, Room 157

T U

E S

D A Y

96