Biophysical Society 66th Annual Meeting Program Guide

Exhibitor Presentation LUMICKS 11:30 am - 1:00 pm, Esplanade, Room 157

Exhibitor Presentation Fluidic Analytics 12:30 pm - 2:00 pm, Esplanade, Room 158

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Unlocking Higher Impact Science With the New C-Trap: A Dedicated Dynamic Single-Molecule Tool For Cytoskeletal Surface Assays In order to obtain a full understanding of the fundamental biological processes in the cell, scientists need to have insights into the molecular mechanisms involved. Studying cytoskeletal structure and transport, therefore, requires information on the mechanical properties and dynam - ics of molecular motors and filaments at the single-molecule level. Not having this information often leads to an incomplete understanding of the molecular processes involved and less impactful science. The new LUMICKS C-Trap product line enables scientists to obtain a thorough understanding of cytoskeletal structure and transport – and do this much faster and more reliably than before. Here, we present our latest developments on the LUMICKS C-Trap that have unlocked dynamic single-molecule analysis in this scientific field and are enabling higher impact studies. The optical tweezers have now been fully optimized for near-surface ex - periments. The highest force resolution (3D) and stability enable measur - ing the smallest motor steps, most precise loads of cytoskeletal motors, and filament bending. This is achieved by adding functionalities such as active surface stabilization, accurate near-surface force calibration, axial force detection, and surface assay force feedback. Connecting the mechanical properties to intramolecular dynamics is now also possible by correlating the optical tweezers measurements with single-molecule TIRF and label-free IRM imaging. All come together in a dedicated surface assay workflow that ensures the fastest time to results. Finally, the imaging module of the new LUMICKS C-Trap can be extended with an integrated widefield imaging mode with single fluorophore sensitivity. This makes the new LUMICKS C-Trap the most versatile multi- user tool and enables applications in the study of not just cytoskeletal structure and transport, but also DNA-binding proteins, protein folding, phase separation, and mechanobiology. Join our presentation to learn about the latest developments in dynamic single-molecule technology and what it can do for your next scientific breakthrough. Speaker Evan Gates, Application Scientist, LUMICKS Founding, Establishing, and Maintaining a Research Laboratory at Primarily Undergraduate Institutions 12:00 pm - 1:30 pm, Esplanade, Room 153 Get guidance on founding, establishing, and maintaining a research laboratory at Primarily Undergraduate Institutions. Panelists are faculty members at PUI's who have been successful in their positions and will share their experiences and answer your questions. Speaker Benjamin Stottrup, Augsburg University Bob Rawle, Williams College Sajith Jayasinghe, California State University San Marcos

Introducing Microfluidic Diffusional Sizing (MDS) Technology; Quantify and Characterize Any Protein Interaction – Even In Complex Back- grounds, Even With Challenging Targets Studying protein interactions is of fundamental importance in a wide variety of research fields, including neurobiology, oncology, immunology, structural biology, and molecular biology. The more scientists understand about protein interactions, the more we will know about the inner workings of a cell, and crucially, the better we will understand what happens when normal cellular function is subverted in human disease. But, with a multitude of different technologies on the market, how do researchers know which technique is right for them? Microfluidic Diffusion Sizing technology (MDS) brings a new tool to the analytical characterization toolbox: a different approach that enables the analysis of protein interactions close to in vivo conditions. Measuring What Matters Scientists can now determine affinity of interaction (KD), concentration of bound protein, and stoichiometric information, in solution, and all in a single experiment, whether using simple biological buffer systems or complex backgrounds such as serum, plasma, saliva, and cell lysates. The MDS technology enables quantification and characterization of any protein interaction – even in complex backgrounds or with challenging targets: • Characterize polyclonal antibodies • Track functional immune response in serum samples • Reveal therapeutic antibody / protein interaction mechanisms • Characterize disordered proteins or higher-order complexes under close to in vivo conditions • Explore aggregation effects and distinguish between specific and non-specific binding During our presentation we will describe a series of case studies and col - laborations with pioneering researchers covering such topics: • Elucidating mechanism of action of Aducanumab in Alzheimer’s Disease • A collaboration with Professor Sara Linse, University of Lund, Swe - den • MDS enables the investigation of viral variants in infectious disease; • A collaboration with Professor Akiko Iwasaki, Yale University School of Medicine Howard Hughes Medical Institute • Characterizing the immune response to viral infections: • A collaboration with Professor Adriano Aguzzi University Hospital Zurich and Professor Tuomas Knowles University of Cambridge • In-Solution Affinity Measurement of a Drug-Induced Protein Com - plex Speaker Molly Coseno, Field Application Specialist, NE, Fluidic Analytics Exhibitor Presentation Chroma Technology 1:30 pm - 3:00 pm, Esplanade, Room 157 Objective Thinking Today’s off-the-shelf microscopes are technological wonders, capable of imaging in many different modalities across a wide range of objective lenses. But what if your need is more defined? This presentation will introduce you to the openFrame: an imaging platform based on optimiz - ing for specific application(s) around the most important optic in your system: the objective lens.

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