Biophysical Society 66th Annual Meeting Program Guide

Public Affairs Committee Meeting 12:00 pm - 1:30 pm, South, Level Three, Room 312 Exhibitor Presentation Fluxion Biosciences 12:30 pm - 2:00 pm, Esplanade, Room 158

Exhibitor Presentation Mad City Labs Inc 1:30 pm - 3:00 pm, Esplanade, Room 157 Novel Single Molecule Methods for Tracking, Transport, and Protein Complex Analyses Capturing the Early Stages of the Virus-Cell Interaction With Active- Feedback Single-Virus Tracking Kevin Welsher Here we introduce an active-feedback 3D microscopy technique capture the dynamics of rapidly diffusing single molecules in solution (3D Single- Molecule Active Real-time Tracking or 3D-SMART). This method “locks" target fluorophores in the focal volume of an optical microscope using real-time feedback to move the sample and compensate for molecular diffusion. 3D-SMART has been successfully applied to single proteins and nucleic acids at diffusive speeds up to 10 μm2/s. We will further describe how this microscope, when combined with rapid volumetric imaging, can capture the early events in the interactions between single viral particles and live cells in three dimensions with millisecond or better temporal resolution. Measuring Activity of Single Transporters in Single Vesicles Using TIRF Microscopy Joseph Mindell Secondary active transporters are essential contributors to ion and sub - strate fluxes across biological membranes. One limitation to our current knowledge is that transporter activity measurements generally reflect the average behavior of a multitude of proteins, while the individual behav - iors of single transporters are unresolved. Here we report development of a novel, single-liposome assay using single-molecule techniques to measure the activity of individual transporters using ClC-ec1, a member of the CLC family of Cl-/H+ exchangers as a model system. Using this method, we observe transport catalyzed by single CLC-ec1 dimers and investigate the nature of stoichiometric coupling in this antiporter. Rapid Extraction and Kinetic Analysis of Protein Complexes From Single Cells Sena Sarıkaya & Dan Dickinson Understanding assembly of molecular machines in developing cells requires a quantitative, biochemical, and in vivo approach. We developed an assay to interrogate the abundance, dynamics, and stability of native protein complexes extracted from single cells. We optically lysed Cae - norhabditis elegans zygotes, captured in vivo single protein complexes on a coverslip using antibodies, and monitored the dynamics of these complexes over time using multi-color MicroMirror Total Internal Reflec - tion Fluorescence microscopy. We developed open-source software to process thousands of kinetic measurements per cell. This assay provides an unprecedented level of resolution as compared to traditional in vitro biochemistry and can be applied to any protein pair that can be labeled and detected. Speakers Joseph Mindell, National Institute of Neurological Disorders and Stroke, NIH Sena Sarıkaya, Department of Molecular Biosciences, The University of Texas at Austin Kevin Welsher, Department of Chemistry, Duke University Meet the Editors, Biophysical Journal 1:45 pm - 3:00 pm, South Lobby/Publications Booth Take this opportunity to meet editors of Biophysical Journal . Editors will be available to talk about the journal and answer questions about where you should submit your work, what will help to get your work published, and what you can expect when you submit. Editor-in-Chief Vasanthi Jayaraman and Editors Meyer Jackson, Scott Showalter, and others will attend.

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From Immunology to Ion Channels: Microfluidic Approaches to Auto- mated Patch Clamp and Cellular Analysis. A Look at IonFlux Mercury and BioFlux Systems from Fluxion Biosciences. Leveraging proprietary microfluidic approaches, Fluxion Biosciences provides unique solutions that simplify and automate complex cell-based assays. This presentation will cover two Fluxion systems used extensively in biophysical characterization of cells: IonFlux and BioFlux. IonFlux Mercury automated patch clamp (APC) systems are capable of recording from 16 to 256 separate cells simultaneously in whole cell patch clamp mode. With unique features such as industry-leading fast in - Plate compound exchange and continuous solution flow, IonFlux systems are used globally in pharma and academic laboratories for both ligand and voltage-gated ion channel screening. Recent developments include automated IC/EC50 calculation workflows, and the introduction of a new range of GABA cells for complete sample-to-result analysis. The BioFlux system is the world’s leading platform for analyzing cell- cell interactions in a flow-controlled environment. Applications include characterization of cell migration, adhesion strength, transmigration, and chemotaxis. BioFlux assays for research and drug development in im - munology, vascular biology, and cancer will be reviewed. Recent research in covid-induced thrombocytopenia and functional analysis of CAR-T cells will be highlighted. Speakers Jeff Jensen, Chief Executive Officer, Fluxion Biosciences Ali Yehia, Chief Scientific Officer, Fluxion Biosciences Education and Career Opportunities Fair 1:00 pm - 3:00 pm, Exhibit Hall ABC This fair will provide opportunities for candidates to meet with represen - tatives from educational institutions as well as industry and government agencies. Students and postdoctoral candidates will be able to meet with representatives from colleges and universities with leading programs in biophysics. Attendees can connect with representatives from industry and agencies who will provide information about employment and fund - ing opportunities at their institutions/companies. Stop by the fair to learn about the variety of opportunities available and to talk one-on-one with representatives from participating organizations. All those attending the Annual Meeting are encouraged to attend.

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